firefly luciferase activity quantification kit Search Results


firefly luciferase based sensitive atp determination kit  (Biaffin Inc)
90
Biaffin Inc firefly luciferase based sensitive atp determination kit
Firefly Luciferase Based Sensitive Atp Determination Kit, supplied by Biaffin Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/firefly+luciferase+activity+quantification+kit/luciferase+based+atp+determination+kit/pmc08275445-227-4-7
Average 90 stars, based on 1 article reviews
firefly luciferase based sensitive atp determination kit - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
dual luciferase reporter assay kit  (Genecopoeia)
96
Genecopoeia dual luciferase reporter assay kit
NFIL3 promotes the transcription of REDD1. (A) mRNA expression of REDD1 determined by RT-qPCR in the peripheral blood neutrophils of 28 gout patients and 20 healthy controls. *** p < 0.001, compared to healthy controls. (B) Western blot analysis of REDD1 protein in the peripheral blood neutrophils of 28 gout patients and 20 healthy controls. *** p < 0.001, compared to healthy controls. (C) mRNA expression of REDD1 determined by RT-qPCR in MSU-treated neutrophils. ** p < 0.01, compared to PBS-treated neutrophils. (D) Western blot analysis of REDD1 protein in MSU-treated neutrophils. ** p < 0.01, compared to PBS-treated neutrophils. (E) The enrichment of NFIL3 in the REDD1 promoter region detected by ChIP assay. ** p < 0.01, compared to IgG antibody. (F) A schematic diagram of NFIL3 binding sites in the REDD1 promoter region. (G) Binding of NFIL3 to the REDD1 promoter analyzed by <t>dual-luciferase</t> reporter assay. *** p < 0.001, compared to cells treated with pGL3-REDD1 + oe-NC. (H) mRNA expression of NFIL3 and REDD1 determined by RT-qPCR in neutrophils following NFIL3 silencing or overexpression. ** p < 0.01 or *** p < 0.001, compared to cells treated with sh-NC. ### p < 0.001, compared to cells treated with Ad-NC. (I) Western blot analysis of NFIL3 and REDD1 proteins in neutrophils following NFIL3 silencing or overexpression. ** p < 0.01 or *** p < 0.001, compared to cells treated with sh-NC. ## p < 0.01 or ### p < 0.001, compared to cells treated with Ad-NC. Data are shown as mean ± standard deviation of three technical replicates. Data between two groups were compared using unpaired t -test while those among multiple groups were assessed by one-way ANOVA.
Dual Luciferase Reporter Assay Kit, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/firefly+luciferase+activity+quantification+kit/Luc-Pair+Firefly+Luciferase+HS+Assay+Kits/pmc08514722-71-12-18
Average 96 stars, based on 1 article reviews
dual luciferase reporter assay kit - by Bioz Stars, 2026-10
96/100 stars
  Buy from Supplier
luc-pair firefly luciferase hs assay kits  (Genecopoeia)
96
Genecopoeia luc-pair firefly luciferase hs assay kits
NFIL3 promotes the transcription of REDD1. (A) mRNA expression of REDD1 determined by RT-qPCR in the peripheral blood neutrophils of 28 gout patients and 20 healthy controls. *** p < 0.001, compared to healthy controls. (B) Western blot analysis of REDD1 protein in the peripheral blood neutrophils of 28 gout patients and 20 healthy controls. *** p < 0.001, compared to healthy controls. (C) mRNA expression of REDD1 determined by RT-qPCR in MSU-treated neutrophils. ** p < 0.01, compared to PBS-treated neutrophils. (D) Western blot analysis of REDD1 protein in MSU-treated neutrophils. ** p < 0.01, compared to PBS-treated neutrophils. (E) The enrichment of NFIL3 in the REDD1 promoter region detected by ChIP assay. ** p < 0.01, compared to IgG antibody. (F) A schematic diagram of NFIL3 binding sites in the REDD1 promoter region. (G) Binding of NFIL3 to the REDD1 promoter analyzed by <t>dual-luciferase</t> reporter assay. *** p < 0.001, compared to cells treated with pGL3-REDD1 + oe-NC. (H) mRNA expression of NFIL3 and REDD1 determined by RT-qPCR in neutrophils following NFIL3 silencing or overexpression. ** p < 0.01 or *** p < 0.001, compared to cells treated with sh-NC. ### p < 0.001, compared to cells treated with Ad-NC. (I) Western blot analysis of NFIL3 and REDD1 proteins in neutrophils following NFIL3 silencing or overexpression. ** p < 0.01 or *** p < 0.001, compared to cells treated with sh-NC. ## p < 0.01 or ### p < 0.001, compared to cells treated with Ad-NC. Data are shown as mean ± standard deviation of three technical replicates. Data between two groups were compared using unpaired t -test while those among multiple groups were assessed by one-way ANOVA.
Luc Pair Firefly Luciferase Hs Assay Kits, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/firefly+luciferase+activity+quantification+kit/Luc-Pair+Firefly+Luciferase+HS+Assay+Kits/custom%40lf007%4010%2E1101%2F2022%2E09%2E02%2E506438
Average 96 stars, based on 1 article reviews
luc-pair firefly luciferase hs assay kits - by Bioz Stars, 2026-10
96/100 stars
  Buy from Supplier
dual luciferase (firefly and renilla) assay kit  (Promega)
90
Promega dual luciferase (firefly and renilla) assay kit
NFIL3 promotes the transcription of REDD1. (A) mRNA expression of REDD1 determined by RT-qPCR in the peripheral blood neutrophils of 28 gout patients and 20 healthy controls. *** p < 0.001, compared to healthy controls. (B) Western blot analysis of REDD1 protein in the peripheral blood neutrophils of 28 gout patients and 20 healthy controls. *** p < 0.001, compared to healthy controls. (C) mRNA expression of REDD1 determined by RT-qPCR in MSU-treated neutrophils. ** p < 0.01, compared to PBS-treated neutrophils. (D) Western blot analysis of REDD1 protein in MSU-treated neutrophils. ** p < 0.01, compared to PBS-treated neutrophils. (E) The enrichment of NFIL3 in the REDD1 promoter region detected by ChIP assay. ** p < 0.01, compared to IgG antibody. (F) A schematic diagram of NFIL3 binding sites in the REDD1 promoter region. (G) Binding of NFIL3 to the REDD1 promoter analyzed by <t>dual-luciferase</t> reporter assay. *** p < 0.001, compared to cells treated with pGL3-REDD1 + oe-NC. (H) mRNA expression of NFIL3 and REDD1 determined by RT-qPCR in neutrophils following NFIL3 silencing or overexpression. ** p < 0.01 or *** p < 0.001, compared to cells treated with sh-NC. ### p < 0.001, compared to cells treated with Ad-NC. (I) Western blot analysis of NFIL3 and REDD1 proteins in neutrophils following NFIL3 silencing or overexpression. ** p < 0.01 or *** p < 0.001, compared to cells treated with sh-NC. ## p < 0.01 or ### p < 0.001, compared to cells treated with Ad-NC. Data are shown as mean ± standard deviation of three technical replicates. Data between two groups were compared using unpaired t -test while those among multiple groups were assessed by one-way ANOVA.
Dual Luciferase (Firefly And Renilla) Assay Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/firefly+luciferase+activity+quantification+kit/luciferase+assay+system/10__1021_slash_acs__jmedchem__1c00439-83-11-17
Average 90 stars, based on 1 article reviews
dual luciferase (firefly and renilla) assay kit - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
single tube assay kit  (Biotium)
95
Biotium single tube assay kit
NFIL3 promotes the transcription of REDD1. (A) mRNA expression of REDD1 determined by RT-qPCR in the peripheral blood neutrophils of 28 gout patients and 20 healthy controls. *** p < 0.001, compared to healthy controls. (B) Western blot analysis of REDD1 protein in the peripheral blood neutrophils of 28 gout patients and 20 healthy controls. *** p < 0.001, compared to healthy controls. (C) mRNA expression of REDD1 determined by RT-qPCR in MSU-treated neutrophils. ** p < 0.01, compared to PBS-treated neutrophils. (D) Western blot analysis of REDD1 protein in MSU-treated neutrophils. ** p < 0.01, compared to PBS-treated neutrophils. (E) The enrichment of NFIL3 in the REDD1 promoter region detected by ChIP assay. ** p < 0.01, compared to IgG antibody. (F) A schematic diagram of NFIL3 binding sites in the REDD1 promoter region. (G) Binding of NFIL3 to the REDD1 promoter analyzed by <t>dual-luciferase</t> reporter assay. *** p < 0.001, compared to cells treated with pGL3-REDD1 + oe-NC. (H) mRNA expression of NFIL3 and REDD1 determined by RT-qPCR in neutrophils following NFIL3 silencing or overexpression. ** p < 0.01 or *** p < 0.001, compared to cells treated with sh-NC. ### p < 0.001, compared to cells treated with Ad-NC. (I) Western blot analysis of NFIL3 and REDD1 proteins in neutrophils following NFIL3 silencing or overexpression. ** p < 0.01 or *** p < 0.001, compared to cells treated with sh-NC. ## p < 0.01 or ### p < 0.001, compared to cells treated with Ad-NC. Data are shown as mean ± standard deviation of three technical replicates. Data between two groups were compared using unpaired t -test while those among multiple groups were assessed by one-way ANOVA.
Single Tube Assay Kit, supplied by Biotium, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/firefly+luciferase+activity+quantification+kit/Firefly+%26+Renilla+Luciferase+Single+Tube+Assay+Kit/pm39810614-195-12-16
Average 95 stars, based on 1 article reviews
single tube assay kit - by Bioz Stars, 2026-10
95/100 stars
  Buy from Supplier
dual luciferase firefly renilla assay system  (BPS Bioscience)
94
BPS Bioscience dual luciferase firefly renilla assay system
NFIL3 promotes the transcription of REDD1. (A) mRNA expression of REDD1 determined by RT-qPCR in the peripheral blood neutrophils of 28 gout patients and 20 healthy controls. *** p < 0.001, compared to healthy controls. (B) Western blot analysis of REDD1 protein in the peripheral blood neutrophils of 28 gout patients and 20 healthy controls. *** p < 0.001, compared to healthy controls. (C) mRNA expression of REDD1 determined by RT-qPCR in MSU-treated neutrophils. ** p < 0.01, compared to PBS-treated neutrophils. (D) Western blot analysis of REDD1 protein in MSU-treated neutrophils. ** p < 0.01, compared to PBS-treated neutrophils. (E) The enrichment of NFIL3 in the REDD1 promoter region detected by ChIP assay. ** p < 0.01, compared to IgG antibody. (F) A schematic diagram of NFIL3 binding sites in the REDD1 promoter region. (G) Binding of NFIL3 to the REDD1 promoter analyzed by <t>dual-luciferase</t> reporter assay. *** p < 0.001, compared to cells treated with pGL3-REDD1 + oe-NC. (H) mRNA expression of NFIL3 and REDD1 determined by RT-qPCR in neutrophils following NFIL3 silencing or overexpression. ** p < 0.01 or *** p < 0.001, compared to cells treated with sh-NC. ### p < 0.001, compared to cells treated with Ad-NC. (I) Western blot analysis of NFIL3 and REDD1 proteins in neutrophils following NFIL3 silencing or overexpression. ** p < 0.01 or *** p < 0.001, compared to cells treated with sh-NC. ## p < 0.01 or ### p < 0.001, compared to cells treated with Ad-NC. Data are shown as mean ± standard deviation of three technical replicates. Data between two groups were compared using unpaired t -test while those among multiple groups were assessed by one-way ANOVA.
Dual Luciferase Firefly Renilla Assay System, supplied by BPS Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/firefly+luciferase+activity+quantification+kit/Dual+Luciferase+(Firefly-Renilla)+Assay+System/pmc09501368-56-22-27
Average 94 stars, based on 1 article reviews
dual luciferase firefly renilla assay system - by Bioz Stars, 2026-10
94/100 stars
  Buy from Supplier
firefly luciferase assay kit 2 0  (Biotium)
94
Biotium firefly luciferase assay kit 2 0
NFIL3 promotes the transcription of REDD1. (A) mRNA expression of REDD1 determined by RT-qPCR in the peripheral blood neutrophils of 28 gout patients and 20 healthy controls. *** p < 0.001, compared to healthy controls. (B) Western blot analysis of REDD1 protein in the peripheral blood neutrophils of 28 gout patients and 20 healthy controls. *** p < 0.001, compared to healthy controls. (C) mRNA expression of REDD1 determined by RT-qPCR in MSU-treated neutrophils. ** p < 0.01, compared to PBS-treated neutrophils. (D) Western blot analysis of REDD1 protein in MSU-treated neutrophils. ** p < 0.01, compared to PBS-treated neutrophils. (E) The enrichment of NFIL3 in the REDD1 promoter region detected by ChIP assay. ** p < 0.01, compared to IgG antibody. (F) A schematic diagram of NFIL3 binding sites in the REDD1 promoter region. (G) Binding of NFIL3 to the REDD1 promoter analyzed by <t>dual-luciferase</t> reporter assay. *** p < 0.001, compared to cells treated with pGL3-REDD1 + oe-NC. (H) mRNA expression of NFIL3 and REDD1 determined by RT-qPCR in neutrophils following NFIL3 silencing or overexpression. ** p < 0.01 or *** p < 0.001, compared to cells treated with sh-NC. ### p < 0.001, compared to cells treated with Ad-NC. (I) Western blot analysis of NFIL3 and REDD1 proteins in neutrophils following NFIL3 silencing or overexpression. ** p < 0.01 or *** p < 0.001, compared to cells treated with sh-NC. ## p < 0.01 or ### p < 0.001, compared to cells treated with Ad-NC. Data are shown as mean ± standard deviation of three technical replicates. Data between two groups were compared using unpaired t -test while those among multiple groups were assessed by one-way ANOVA.
Firefly Luciferase Assay Kit 2 0, supplied by Biotium, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/firefly+luciferase+activity+quantification+kit/Firefly+Luciferase+Assay+Kit+2%2E0/pmc09081475-78-1-6
Average 94 stars, based on 1 article reviews
firefly luciferase assay kit 2 0 - by Bioz Stars, 2026-10
94/100 stars
  Buy from Supplier
enhanced luciferase assay kit  (Becton Dickinson)
90
Becton Dickinson enhanced luciferase assay kit
NFIL3 promotes the transcription of REDD1. (A) mRNA expression of REDD1 determined by RT-qPCR in the peripheral blood neutrophils of 28 gout patients and 20 healthy controls. *** p < 0.001, compared to healthy controls. (B) Western blot analysis of REDD1 protein in the peripheral blood neutrophils of 28 gout patients and 20 healthy controls. *** p < 0.001, compared to healthy controls. (C) mRNA expression of REDD1 determined by RT-qPCR in MSU-treated neutrophils. ** p < 0.01, compared to PBS-treated neutrophils. (D) Western blot analysis of REDD1 protein in MSU-treated neutrophils. ** p < 0.01, compared to PBS-treated neutrophils. (E) The enrichment of NFIL3 in the REDD1 promoter region detected by ChIP assay. ** p < 0.01, compared to IgG antibody. (F) A schematic diagram of NFIL3 binding sites in the REDD1 promoter region. (G) Binding of NFIL3 to the REDD1 promoter analyzed by <t>dual-luciferase</t> reporter assay. *** p < 0.001, compared to cells treated with pGL3-REDD1 + oe-NC. (H) mRNA expression of NFIL3 and REDD1 determined by RT-qPCR in neutrophils following NFIL3 silencing or overexpression. ** p < 0.01 or *** p < 0.001, compared to cells treated with sh-NC. ### p < 0.001, compared to cells treated with Ad-NC. (I) Western blot analysis of NFIL3 and REDD1 proteins in neutrophils following NFIL3 silencing or overexpression. ** p < 0.01 or *** p < 0.001, compared to cells treated with sh-NC. ## p < 0.01 or ### p < 0.001, compared to cells treated with Ad-NC. Data are shown as mean ± standard deviation of three technical replicates. Data between two groups were compared using unpaired t -test while those among multiple groups were assessed by one-way ANOVA.
Enhanced Luciferase Assay Kit, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/firefly+luciferase+activity+quantification+kit/enhanced+luciferase+assay+kit/pmc03193578-155-11-14
Average 90 stars, based on 1 article reviews
enhanced luciferase assay kit - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
dual firefly/renilla luciferase reporter kit  (Promega)
90
Promega dual firefly/renilla luciferase reporter kit
NFIL3 promotes the transcription of REDD1. (A) mRNA expression of REDD1 determined by RT-qPCR in the peripheral blood neutrophils of 28 gout patients and 20 healthy controls. *** p < 0.001, compared to healthy controls. (B) Western blot analysis of REDD1 protein in the peripheral blood neutrophils of 28 gout patients and 20 healthy controls. *** p < 0.001, compared to healthy controls. (C) mRNA expression of REDD1 determined by RT-qPCR in MSU-treated neutrophils. ** p < 0.01, compared to PBS-treated neutrophils. (D) Western blot analysis of REDD1 protein in MSU-treated neutrophils. ** p < 0.01, compared to PBS-treated neutrophils. (E) The enrichment of NFIL3 in the REDD1 promoter region detected by ChIP assay. ** p < 0.01, compared to IgG antibody. (F) A schematic diagram of NFIL3 binding sites in the REDD1 promoter region. (G) Binding of NFIL3 to the REDD1 promoter analyzed by <t>dual-luciferase</t> reporter assay. *** p < 0.001, compared to cells treated with pGL3-REDD1 + oe-NC. (H) mRNA expression of NFIL3 and REDD1 determined by RT-qPCR in neutrophils following NFIL3 silencing or overexpression. ** p < 0.01 or *** p < 0.001, compared to cells treated with sh-NC. ### p < 0.001, compared to cells treated with Ad-NC. (I) Western blot analysis of NFIL3 and REDD1 proteins in neutrophils following NFIL3 silencing or overexpression. ** p < 0.01 or *** p < 0.001, compared to cells treated with sh-NC. ## p < 0.01 or ### p < 0.001, compared to cells treated with Ad-NC. Data are shown as mean ± standard deviation of three technical replicates. Data between two groups were compared using unpaired t -test while those among multiple groups were assessed by one-way ANOVA.
Dual Firefly/Renilla Luciferase Reporter Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/firefly+luciferase+activity+quantification+kit/dual+luciferase+reporter+assay+kit/us08318135-499-7-15
Average 90 stars, based on 1 article reviews
dual firefly/renilla luciferase reporter kit - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
firefly luciferase assay kit  (Biotium)
95
Biotium firefly luciferase assay kit
NFIL3 promotes the transcription of REDD1. (A) mRNA expression of REDD1 determined by RT-qPCR in the peripheral blood neutrophils of 28 gout patients and 20 healthy controls. *** p < 0.001, compared to healthy controls. (B) Western blot analysis of REDD1 protein in the peripheral blood neutrophils of 28 gout patients and 20 healthy controls. *** p < 0.001, compared to healthy controls. (C) mRNA expression of REDD1 determined by RT-qPCR in MSU-treated neutrophils. ** p < 0.01, compared to PBS-treated neutrophils. (D) Western blot analysis of REDD1 protein in MSU-treated neutrophils. ** p < 0.01, compared to PBS-treated neutrophils. (E) The enrichment of NFIL3 in the REDD1 promoter region detected by ChIP assay. ** p < 0.01, compared to IgG antibody. (F) A schematic diagram of NFIL3 binding sites in the REDD1 promoter region. (G) Binding of NFIL3 to the REDD1 promoter analyzed by <t>dual-luciferase</t> reporter assay. *** p < 0.001, compared to cells treated with pGL3-REDD1 + oe-NC. (H) mRNA expression of NFIL3 and REDD1 determined by RT-qPCR in neutrophils following NFIL3 silencing or overexpression. ** p < 0.01 or *** p < 0.001, compared to cells treated with sh-NC. ### p < 0.001, compared to cells treated with Ad-NC. (I) Western blot analysis of NFIL3 and REDD1 proteins in neutrophils following NFIL3 silencing or overexpression. ** p < 0.01 or *** p < 0.001, compared to cells treated with sh-NC. ## p < 0.01 or ### p < 0.001, compared to cells treated with Ad-NC. Data are shown as mean ± standard deviation of three technical replicates. Data between two groups were compared using unpaired t -test while those among multiple groups were assessed by one-way ANOVA.
Firefly Luciferase Assay Kit, supplied by Biotium, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/firefly+luciferase+activity+quantification+kit/Firefly+Luciferase+Assay+Kit/pmc10058554-108-22-26
Average 95 stars, based on 1 article reviews
firefly luciferase assay kit - by Bioz Stars, 2026-10
95/100 stars
  Buy from Supplier
firefly renilla luciferase reporter gene assay kit  (Guangzhou Biolight Biotechnology Co Ltd)
86
Guangzhou Biolight Biotechnology Co Ltd firefly renilla luciferase reporter gene assay kit
NFIL3 promotes the transcription of REDD1. (A) mRNA expression of REDD1 determined by RT-qPCR in the peripheral blood neutrophils of 28 gout patients and 20 healthy controls. *** p < 0.001, compared to healthy controls. (B) Western blot analysis of REDD1 protein in the peripheral blood neutrophils of 28 gout patients and 20 healthy controls. *** p < 0.001, compared to healthy controls. (C) mRNA expression of REDD1 determined by RT-qPCR in MSU-treated neutrophils. ** p < 0.01, compared to PBS-treated neutrophils. (D) Western blot analysis of REDD1 protein in MSU-treated neutrophils. ** p < 0.01, compared to PBS-treated neutrophils. (E) The enrichment of NFIL3 in the REDD1 promoter region detected by ChIP assay. ** p < 0.01, compared to IgG antibody. (F) A schematic diagram of NFIL3 binding sites in the REDD1 promoter region. (G) Binding of NFIL3 to the REDD1 promoter analyzed by <t>dual-luciferase</t> reporter assay. *** p < 0.001, compared to cells treated with pGL3-REDD1 + oe-NC. (H) mRNA expression of NFIL3 and REDD1 determined by RT-qPCR in neutrophils following NFIL3 silencing or overexpression. ** p < 0.01 or *** p < 0.001, compared to cells treated with sh-NC. ### p < 0.001, compared to cells treated with Ad-NC. (I) Western blot analysis of NFIL3 and REDD1 proteins in neutrophils following NFIL3 silencing or overexpression. ** p < 0.01 or *** p < 0.001, compared to cells treated with sh-NC. ## p < 0.01 or ### p < 0.001, compared to cells treated with Ad-NC. Data are shown as mean ± standard deviation of three technical replicates. Data between two groups were compared using unpaired t -test while those among multiple groups were assessed by one-way ANOVA.
Firefly Renilla Luciferase Reporter Gene Assay Kit, supplied by Guangzhou Biolight Biotechnology Co Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/firefly+luciferase+activity+quantification+kit/assay+firefly+gene+kit+luciferase+renilla+reporter/10__7150_slash_ijbs__125454-223-9-17
Average 86 stars, based on 1 article reviews
firefly renilla luciferase reporter gene assay kit - by Bioz Stars, 2026-10
86/100 stars
  Buy from Supplier
eppendorf tube  (Eppendorf AG)
99
Eppendorf AG eppendorf tube
NFIL3 promotes the transcription of REDD1. (A) mRNA expression of REDD1 determined by RT-qPCR in the peripheral blood neutrophils of 28 gout patients and 20 healthy controls. *** p < 0.001, compared to healthy controls. (B) Western blot analysis of REDD1 protein in the peripheral blood neutrophils of 28 gout patients and 20 healthy controls. *** p < 0.001, compared to healthy controls. (C) mRNA expression of REDD1 determined by RT-qPCR in MSU-treated neutrophils. ** p < 0.01, compared to PBS-treated neutrophils. (D) Western blot analysis of REDD1 protein in MSU-treated neutrophils. ** p < 0.01, compared to PBS-treated neutrophils. (E) The enrichment of NFIL3 in the REDD1 promoter region detected by ChIP assay. ** p < 0.01, compared to IgG antibody. (F) A schematic diagram of NFIL3 binding sites in the REDD1 promoter region. (G) Binding of NFIL3 to the REDD1 promoter analyzed by <t>dual-luciferase</t> reporter assay. *** p < 0.001, compared to cells treated with pGL3-REDD1 + oe-NC. (H) mRNA expression of NFIL3 and REDD1 determined by RT-qPCR in neutrophils following NFIL3 silencing or overexpression. ** p < 0.01 or *** p < 0.001, compared to cells treated with sh-NC. ### p < 0.001, compared to cells treated with Ad-NC. (I) Western blot analysis of NFIL3 and REDD1 proteins in neutrophils following NFIL3 silencing or overexpression. ** p < 0.01 or *** p < 0.001, compared to cells treated with sh-NC. ## p < 0.01 or ### p < 0.001, compared to cells treated with Ad-NC. Data are shown as mean ± standard deviation of three technical replicates. Data between two groups were compared using unpaired t -test while those among multiple groups were assessed by one-way ANOVA.
Eppendorf Tube, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/firefly+luciferase+activity+quantification+kit/Eppendorf/pmc10902212-102-41-41
Average 99 stars, based on 1 article reviews
eppendorf tube - by Bioz Stars, 2026-10
99/100 stars
  Buy from Supplier

Image Search Results


NFIL3 promotes the transcription of REDD1. (A) mRNA expression of REDD1 determined by RT-qPCR in the peripheral blood neutrophils of 28 gout patients and 20 healthy controls. *** p < 0.001, compared to healthy controls. (B) Western blot analysis of REDD1 protein in the peripheral blood neutrophils of 28 gout patients and 20 healthy controls. *** p < 0.001, compared to healthy controls. (C) mRNA expression of REDD1 determined by RT-qPCR in MSU-treated neutrophils. ** p < 0.01, compared to PBS-treated neutrophils. (D) Western blot analysis of REDD1 protein in MSU-treated neutrophils. ** p < 0.01, compared to PBS-treated neutrophils. (E) The enrichment of NFIL3 in the REDD1 promoter region detected by ChIP assay. ** p < 0.01, compared to IgG antibody. (F) A schematic diagram of NFIL3 binding sites in the REDD1 promoter region. (G) Binding of NFIL3 to the REDD1 promoter analyzed by dual-luciferase reporter assay. *** p < 0.001, compared to cells treated with pGL3-REDD1 + oe-NC. (H) mRNA expression of NFIL3 and REDD1 determined by RT-qPCR in neutrophils following NFIL3 silencing or overexpression. ** p < 0.01 or *** p < 0.001, compared to cells treated with sh-NC. ### p < 0.001, compared to cells treated with Ad-NC. (I) Western blot analysis of NFIL3 and REDD1 proteins in neutrophils following NFIL3 silencing or overexpression. ** p < 0.01 or *** p < 0.001, compared to cells treated with sh-NC. ## p < 0.01 or ### p < 0.001, compared to cells treated with Ad-NC. Data are shown as mean ± standard deviation of three technical replicates. Data between two groups were compared using unpaired t -test while those among multiple groups were assessed by one-way ANOVA.

Journal: Frontiers in Medicine

Article Title: NFIL3 Facilitates Neutrophil Autophagy, Neutrophil Extracellular Trap Formation and Inflammation During Gout via REDD1-Dependent mTOR Inactivation

doi: 10.3389/fmed.2021.692781

Figure Lengend Snippet: NFIL3 promotes the transcription of REDD1. (A) mRNA expression of REDD1 determined by RT-qPCR in the peripheral blood neutrophils of 28 gout patients and 20 healthy controls. *** p < 0.001, compared to healthy controls. (B) Western blot analysis of REDD1 protein in the peripheral blood neutrophils of 28 gout patients and 20 healthy controls. *** p < 0.001, compared to healthy controls. (C) mRNA expression of REDD1 determined by RT-qPCR in MSU-treated neutrophils. ** p < 0.01, compared to PBS-treated neutrophils. (D) Western blot analysis of REDD1 protein in MSU-treated neutrophils. ** p < 0.01, compared to PBS-treated neutrophils. (E) The enrichment of NFIL3 in the REDD1 promoter region detected by ChIP assay. ** p < 0.01, compared to IgG antibody. (F) A schematic diagram of NFIL3 binding sites in the REDD1 promoter region. (G) Binding of NFIL3 to the REDD1 promoter analyzed by dual-luciferase reporter assay. *** p < 0.001, compared to cells treated with pGL3-REDD1 + oe-NC. (H) mRNA expression of NFIL3 and REDD1 determined by RT-qPCR in neutrophils following NFIL3 silencing or overexpression. ** p < 0.01 or *** p < 0.001, compared to cells treated with sh-NC. ### p < 0.001, compared to cells treated with Ad-NC. (I) Western blot analysis of NFIL3 and REDD1 proteins in neutrophils following NFIL3 silencing or overexpression. ** p < 0.01 or *** p < 0.001, compared to cells treated with sh-NC. ## p < 0.01 or ### p < 0.001, compared to cells treated with Ad-NC. Data are shown as mean ± standard deviation of three technical replicates. Data between two groups were compared using unpaired t -test while those among multiple groups were assessed by one-way ANOVA.

Article Snippet: After 48 h of culture, the luciferase activity was determined using a Dual Luciferase Reporter Assay kit (D0010, GeneCopoeia, Beijing Solarbio Science & Technology Co., Ltd., Beijing, China) on a Glomax 20/20 luminometer (E5311, Promega, Zhongmei Biotechnology Co., Ltd., Shannxi, China), which was expressed as the ratio of relative luciferase activity of firefly luciferase to that of renilla luciferase.

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Binding Assay, Luciferase, Reporter Assay, Over Expression, Standard Deviation